PARTIAL ACID HYDROLYSATES OF PROTEINS

Determination of the degree of hydrolysis of partial acid hydrolysates of casein and fibrin.

White and Elman (1) in 1942 described condit,ions for the partial acid hydrolysis of proteins under which most of the tryptophane was retained undamaged. These hydrolysates appeared suitable for intravenous injection, as was reported briefly in the same communication. Studies have since been carried out by Risser, Schenck, and Frost (2) in these laboratories which demonstrate that partial acid ...

Uracil in formic acid hydrolysates of deoxyribonucleic acid.

1. When DNA is hydrolysed with formic acid for 30min. at 175 degrees and the hydrolysate is chromatographed on paper with propan-2-ol-2n-hydrochloric acid, in addition to expected ultraviolet-absorbing spots corresponding to guanine, adenine, cytosine and thymine, an ultraviolet-absorbing region with R(F) similar to that of uracil can be detected. Uracil was separated from this region and ident...

Peptidoglutaminase Deamidation of Proteins and Protein Hydrolysates for Improved Food

The limited deamidating ability of peptidoglutaminase {PGase) toward intact food proteins (0-6% deamidation) can be significantly enhanced by prior protein hydrolysis and altering protein conformation by such means as moist heat. PGase deamidation increases protein solubility and improves emulsifying and other physical properties under mildly acidic conditions. A batch reactor method was develo...

The amino-acid sequence in the phenylalanyl chain of insulin. I. The identification of lower peptides from partial hydrolysates.

The amino-acid sequence in the glycyl chain of insulin. I. The identification of lower peptides from partial hydrolysates.

Insulin has been shown to be composed oftwo types of polypeptide chains held together by -S-bridges. These chains may be separated after breaking the -S-Sbridges by oxidation to -SOH groups (Sanger, 1949 a). The more acidic glycyl chain is found in the fraction (A) which is soluble at pH 6-5 and the more basic phenylalanyl chain in the insoluble fraction (B). Bymaking use ofthe methods of Consd...